![An optimized protocol for stepwise optimization of real-time RT-PCR analysis | Horticulture Research An optimized protocol for stepwise optimization of real-time RT-PCR analysis | Horticulture Research](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fs41438-021-00616-w/MediaObjects/41438_2021_616_Fig1_HTML.png)
An optimized protocol for stepwise optimization of real-time RT-PCR analysis | Horticulture Research
![SOLVED: PartL Fill in the missing information the table below: Hint: Use CIV1 CZVZ calculate volumes needed to dilute stock to working concentration. Component Stock Concentration Working (Final) Concentration Volume Needed for SOLVED: PartL Fill in the missing information the table below: Hint: Use CIV1 CZVZ calculate volumes needed to dilute stock to working concentration. Component Stock Concentration Working (Final) Concentration Volume Needed for](https://cdn.numerade.com/ask_images/e2e71c8ed85b433bbcae2445a783e638.jpg)
SOLVED: PartL Fill in the missing information the table below: Hint: Use CIV1 CZVZ calculate volumes needed to dilute stock to working concentration. Component Stock Concentration Working (Final) Concentration Volume Needed for
![SOLVED: You need to perform PCR to amplify gene for this assignment: You will create PCR solution with 25LLtota volume PCR recipe 10X PCR master mix stock DNA (125 ng total DNA) SOLVED: You need to perform PCR to amplify gene for this assignment: You will create PCR solution with 25LLtota volume PCR recipe 10X PCR master mix stock DNA (125 ng total DNA)](https://cdn.numerade.com/ask_images/04cd2b022c434e5eacbc3306b2f743cf.jpg)
SOLVED: You need to perform PCR to amplify gene for this assignment: You will create PCR solution with 25LLtota volume PCR recipe 10X PCR master mix stock DNA (125 ng total DNA)
![SOLVED: Calculate the stock concentration of each of the PCR reagents that will [ be used next week, as indicat the following table Pay attention to the units of each reagent: (3 SOLVED: Calculate the stock concentration of each of the PCR reagents that will [ be used next week, as indicat the following table Pay attention to the units of each reagent: (3](https://cdn.numerade.com/ask_images/272e87b7be7547628805a1e54dd508d9.jpg)